Florida panthers roam far and wide:
Males need 200 square miles and females around 80 to live.
Southwest Florida's panther population is now 150 large, or so the estimate goes. That means that eventually they run into each other and/or motor vehicles while attempting to cross over roads, especially at night, both of which with lethal result.
Wildlife crossings have been strategically placed to help them safely cross under the roads instead.
As for finding more territory ...
That almost makes highway re-engineering look easy.
Here's an article that explains.